Remove the fermentor gradually with care while the temperature is still warm and tolerable
Add air line with sterilized air to maintain positive pressure within the fermentor. Condensation of steam might lead to negative pressure and suction of contaminants from the surrounding
Use a slow stirrer speed to improve heat loss from fermentor
Leave fermentor standing till its cool enough to be inoculated if not the culture will die. Possible leave the cooling of fermentor in aseptic area or inoculation chamber
Friday, January 4, 2008
POST STERILIZATION ACTIVITIES
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