PROBLEMS OF SAMPLING

Everybody knows why we carry out sampling. We carry out samplings or obtaining samples so that we can have information or data of the system being sampled. The samples obtained are analyzed and the data obtained are used by us so that we can “extrapolate” or “guess” about the composition status of the system being studied.

In certain systems being studied such as fermenters, they are even equipped with specific purpose built sampling ports where samples can be obtained for analyses.

The sampling ports are often designed more for the ease of sampling or aseptic integrity rather than for the purpose of a truly representative sample!

It is very important in any sampling that the samples obtained are truly representative of the system so as to truly reflect the composition of the system. A non truly representative sample will have the tendency to give inaccurate reading. As they often say, the results obtained are as good as the sample itself. Analyses of the sample is only valid for the sample itself and will not truly reflect the status of the system.

Many users of fermentors when using the samplers do not often reflect the weak links in the process of sampling and to put complete trust that the samples they obtained are very accurate and reflecting the real composition of the fermentation process.

It is important that since a lot depend on the samples and sampling process itself that a proper sampling plan be properly prepared to take into account the weaknesses of such samplings.

They need to carry out preliminary studies and validate their sampling procedures so that their data is acceptable and trust worthy. One of the key areas they must look into is the homogeneity of the fermentor content and statistical validity of their sampling


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